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Origin: The Context for this problem statement was shared by Wenyu Gu at a Homeworld workshop on protein engineering and climate tech. It was further explored as part of a collaborative effort between Spark Climate Solutions and Homeworld Collective to identify and share priority problems at the intersection of biotech and atmospheric methane removal.

Contributors: Paul Reginato1, Mary Lidstrom2, Jeremy Semrau3, Jessica Swanson4, Lisa Stein5, Wenyu Gu6, Mark Hansen7, Paige Brocidiacono8, Ariana Caiati9, Erin Wilson10

Lead contact: Paul Reginato ([email protected])

Problem Statement

Motivating Factor

CH4 emissions have contributed ~30% of global warming to date [1], and natural sources may increase via feedback to warming [2]. Technologies for oxidizing atmospheric CH^₄, area CH₄ emissions, and unavoidable point sources could substantially mitigate climate change. While CH₄ above ~44,000 ppm can be flared, ~75% of CH₄ pollution is atmospheric (2 ppm) or area emissions below 1000 ppm that are too dilute to be oxidized at scale using existing technologies [3].

Methane monooxygenase (MMO) enzymes, found in methanotrophic bacteria, naturally catalyze oxidation of CH₄ to methanol in a one-step reaction at ambient conditions [4]. Oxidation of dilute CH₄ at scale may be possible using methanotrophs or cell-free MMO in flow-through reactors; however, substantial efficiency improvements over the state of the art are needed for such reactors to be economical [4][5]. For example, modeling of a reactor system using Methylosinus trichosporium OB3b estimated up to 10-fold cost reduction is necessary to oxidize 500 ppm CH₄ for $100/t CO₂e [5].

Specific Constraint

CH₄ oxidation efficiency improvements may be achieved by discovering or engineering methanotrophs or MMO variants that operate efficiently at low CH₄ concentrations. Such work is underway [6] and there is much more to be done [7] [8][9]. However, researchers lack a clear performance target for biological CH₄ oxidation agents, because there is no available analysis describing the relationship between biological oxidation performance and cost across a range of dilute CH₄ concentrations. Further, methanotroph-based bioreactor systems thus far have generally not been designed to handle CH₄ concentrations below 1000 ppm [10]. Development of reactor designs with associated techno-economic and life-cycle analyses would illuminate the potential for economic feasibility at scale and orient bioengineering of methane oxidation toward scalable targets.

Actionable Goals

Designs should be developed for CH₄ bioreactors that are optimized for low (2-1000 ppm) CH₄ concentration using methanotrophs, mixed microbial cultures, or cell-free MMO. High-level techno-economic and life-cycle analysis should be performed to assess the costs of biological methane oxidation efficiency at scale. For cell-free MMO, the need for reducants to activate O₂ should be addressed by assuming reductants are regenerated electrochemically or that an enzyme system is used that generates reactive oxygen through methane oxidation reactions without other inputs [9].

Rather than treating oxidation efficiency as a constant, modeling should assess the impact of a range of oxidation efficiencies on cost, to provide targets for organism discovery and engineering. The impact of oxidation efficiency on optimal reactor design should also be considered, as well as methods to enhance CH₄ mass transfer. CH₄ oxidation performance should be discussed in terms of specific affinity (a˚_S), defined as V_{max app}/k_M, since a˚_S is known to be a more relevant parameter for enzyme performance at substrate concentrations well below k_M [11].

Additional information

Related problem statements 

• Genetic tools for methanotrophs to manipulate particulate methane monooxygenase

• Discover or engineer efficient soluble methane monooxygenase

• Cell-free methane oxidation without reductant input